In the Biovendor Human Procalcitonin ELISA, standards, superior controls and samples are incubated in microplate wells pre-coated with polyclonal anti-human procalcitonin antibody. After 120 account evolution and washing, biotin labelled polyclonal anti-human procalcitonin antibiotic is added and incubated with captured procalcitonin for 60 minutes. After accession washing, streptavidin-HRP conjugate is added. After 30 account evolution and the endure abrasion step, the actual conjugate is accustomed to acknowledge with the substrate band-aid (TMB). The acknowledgment is chock-full by accession of acerb band-aid and absorbance of the consistent chicken artefact is measured. The absorbance is proportional to the absorption of procalcitonin. A accepted ambit is complete by acute absorbance ethics against procalcitonin concentrations of standards, and concentrations of alien samples are bent application this accepted curve.